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Recombinant human PKLR protein

CAT: 0112-ATGP0714-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0714-250Size:250 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity > 80 units/mg. One unit will convert 1.0 umole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C.
Product Name Alternative
Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
Enzyme Activity, SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity: > 80unit/mg. One unit will convert 1.0 umole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C.
Molecular Weight
59.2 kDa (549aa) confirmed by MALDI-TOF
Additionnal Information
PKLR, Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK, ATGP0714-10 µg, ATGP0714-20 µg, ATGP0714-50 µg, ATGP0714-100 µg, ATGP0714-250 µg, ATGP0714-500 µg, ATGP0714-1 mg, ATGP0714-10, ATGP0714-20, ATGP0714-50, ATGP0714-100, ATGP0714-250, ATGP0714-500, ATGP0714-1
References & Citations
Meza NW., et al. (2009) Mol Ther. 17 (12) :2000-9.; ; Roy MF., et al. (2007) J Exp Med. 204 (12) :2949-61.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_000289
Uniprot Accession Number
P30613
Species
Human
AA Sequence
MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS

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