Human TBCA antibody

CAT: 0112-ATGA0155-050Size: 50 µLDry Ice: NoHazardous: No
CAT#:0112-ATGA0155-050Size:50 µL
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Description
Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest.
Product Name Alternative
Tubulin folding cofactor A, Tubulin folding cofactor A, chaperonin cofactor a, tubulin specific chaperone a, TBCA, Tubulin folding cofactor A CFA, Co chaperonin associated with a & b tubulin, Cofactor A, TCP1 chaperonin cofactor A, Tubulin cofactor a
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant TBCA (1-108aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT1A5
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
TBCA, ATGA0155-10 µg, ATGA0155-20 µg, ATGA0155-50 µg, ATGA0155-100 µg, ATGA0155-250 µg, ATGA0155-500 µg, ATGA0155-1 mg, ATGA0155-10, ATGA0155-20, ATGA0155-50, ATGA0155-100, ATGA0155-250, ATGA0155-500, ATGA0155-1
References & Citations
Nolasco S, et al., (2005) FEBS Lett 579 (17) :3515-3524.; ; Tian G, et al., (1996) Cell 86 (2) :287-296.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cell Cycle
NCBI Accession Number
NP_004598
Uniprot Accession Number
O75347
WB Description
The cell lysate of LNCap (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: K562 cell lysate; ; {ATGA0155-WB2.jpg}
FACS Description
Flow cytometry analysis of TBCA in HeLa cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
IF Description
ICC/IF analysis of TBCA in A549 cells. The cell was stained with ATGA0155 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of TBCA in HeLa cells. The cell was stained with ATGA0155 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010129463677.jpg}

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