Human TBCB antibody

• Description :

  Tubulin folding cofactor B, also known as TBCB, belongs to the Microtubules family. Biosynthesis of functional microtubules involve the participation of several chaperones, termed Tubulin folding cofactors A (TBCA), B (TBCB), D (TBCD), E (TBCE) and C (TBCC), that act on folding intermediates downstream of the cytosolic chaperon, alternatively named TCP. TBCB is a 244 amino acid cytoplasmic protein containing one CAP-Gly domain and in widely expressed. TBCB is involved in the regulation of tubulin heterodimer dissociation and may function as a negative regulator of axonal growth.

• Product Name Alternative :

  Tubulin folding cofactor B, CG22, CKAP1, CKAPI

• Host :

  Mouse

• Antigen Species :

  Human

• Reactivity :

  Human

• Immunogen :

  Recombinant human TBCB (1-244aa) purified from E. coli

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  AT1F6

• Conjugation :

  Unconjugated

• Applications :

  ELISA, WB, FACS, ICC/IF

• Purification Method :

  By protein-A affinity chromatography

• Concentration :

  1 mg/mL (determined by BCA assay)

• Additionnal Information :

  TBCB, Tubulin folding cofactor B, CG22, CKAP1, CKAPI, ATGA0302-10 µg, ATGA0302-20 µg, ATGA0302-50 µg, ATGA0302-100 µg, ATGA0302-250 µg, ATGA0302-500 µg, ATGA0302-1 mg, ATGA0302-10, ATGA0302-20, ATGA0302-50, ATGA0302-100, ATGA0302-250, ATGA0302-500, ATGA0302-1

• References & Citations :

  Kortazar D., et al. (2007) Exp Cell Res. 313:425-436.; ; Tian G., et al. (1997) J Cell Biol. 138: 821-832; ; Fanarraga ML., et al. (2009) Exp cell Res 315:535-541;

• Storage Conditions :

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation :

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes :

  The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1500

• Scientific Category :

  Cytoskeleton

• NCBI Accession Number :

  NP_001272

• Uniprot Accession Number :

  Q99426

• WB Description :

  The cell lysates of Jurkat (40ug) and A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCB antibody (1:1500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• FACS Description :

  Flow cytometry analysis of TBCB in HeLa cells. The cell was stained with ATGA0302 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

• IF Description :

  ICC/IF analysis of TBCB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TBCB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .