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Human eIF-5A1/EIF5A antibody

CAT: 0112-ATGA0128-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0128-050Size:50 µL
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Description
Eukaryotic translation initiation factor 5A (eIF5A) is the only protein Known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer.
Product Name Alternative
Eukaryotic translation initiation factor 5A-1 isoform B, Eukaryotic translation initiation factor 5A, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human EIF5A (1-154aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT4D1
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
EIF5A, ATGA0128-10 µg, ATGA0128-20 µg, ATGA0128-50 µg, ATGA0128-100 µg, ATGA0128-250 µg, ATGA0128-500 µg, ATGA0128-1 mg, ATGA0128-10, ATGA0128-20, ATGA0128-50, ATGA0128-100, ATGA0128-250, ATGA0128-500, ATGA0128-1
References & Citations
Saini P., et al. (2009) Nature. 459 (7243) : 118-21.; ; Gosslau A., et al. (2009) J Cell Physiol. 219 (2) : 485-93.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001961
Uniprot Accession Number
P63241
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF5A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: 293T cell lysate; ; Lane 3.: HeLa cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: NIH-3T3 cell lysate
FACS Description
Flow cytometry analysis of EIF5A in Balb/3T3 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of EIF5A in Balb/3T3 cells. The cell was stained with ATGA0128 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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