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Recombinant human eIF-1A/EIF1 protein

CAT: 0112-ATGP0461-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0461-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Sui1iso1, SuI1, Protein translation factor SUI1 homolog, ISO1, Eukaryotic translation initiation factor 1, EIF1A, EIF-1, EIF1, A121
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
16.9 kDa (150aa) confirmed by MALDI-TOF
Additionnal Information
Sui1iso1, SuI1, Protein translation factor SUI1 homolog, ISO1, Eukaryotic translation initiation factor 1, EIF1A, EIF-1, EIF1, A121, ATGP0461-10 µg, ATGP0461-20 µg, ATGP0461-50 µg, ATGP0461-100 µg, ATGP0461-250 µg, ATGP0461-500 µg, ATGP0461-1 mg, ATGP0461-010, ATGP0461-020, ATGP0461-050, ATGP0461-100, ATGP0461-250, ATGP0461-500, ATGP0461-01M
References & Citations
Dennis MD., et al. (2009) J Biol Chem. 284 (31) :20615-28. ; ; Pisarev AV., et al. (2007) Cell. 131 (2) :286-99.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1 M NaCl, and 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_005792
Uniprot Accession Number
P41567
Species
Human
AA Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMSA IQNLHSFDPF ADASKGDDLL PAGTEDYIHI RIQQRNGRKT LTTVQGIADD YDKKKLVKAF KKKFACNGTV IEHPEYGEVI QLQGDQRKNI CQFLVEIGLA KDDQLKVHGF

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