Recombinant human eIF-5A2/EIF5A2 protein

• Description:

  EIF5A2, a member of eukaryotic initiation factor 5A subfamily, is an essential protein tightly linked to cellular polyamine homeostasis. The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond during the initial stage of protein synthesis. It seems to be the only eukaryotic protein to have a hypusine residue, which is a post-translational modification of a lysine by the addition of a butylamino group. Recombinant human EIF5A2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

• Product Name Alternative:

  MGC124093, MGC124092, Eukaryotic translation initiation factor 5A2, Eukaryotic initiation factor 5A isoform 2, eIF5AII, EIF5A2, eIF-4D

• Expression System:

  E.coli

• Antigen Species:

  Human

• Tag:

  His-Tag

• Applications:

  SDS-PAGE

• Concentration:

  0.5 mg/mL (determined by Bradford assay)

• Purity:

  > 85% by SDS-PAGE

• Molecular Weight:

  18.9 kDa (173aa) confirmed by MALDI-TOF

• Additionnal Information:

  MGC124093, MGC124092, Eukaryotic translation initiation factor 5A2, Eukaryotic initiation factor 5A isoform 2, eIF5AII, EIF5A2, EIF5A 2, eIF-4D, eIF 5A2, eIF 5A 2, ATGP0596-10 µg, ATGP0596-20 µg, ATGP0596-50 µg, ATGP0596-100 µg, ATGP0596-250 µg, ATGP0596-500 µg, ATGP0596-1 mg, ATGP0596-010, ATGP0596-020, ATGP0596-050, ATGP0596-100, ATGP0596-250, ATGP0596-500, ATGP0596-01M

• References & Citations:

  Guan XY., et al. (2010) Hepatology. 51 (4) :1255-63.; ; Luk JM., et al. (2009) Int J Cancer. 127 (4) :968-76.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

• Scientific Category:

  Epigenomics (Transcription & Translation)

• NCBI Accession Number:

  NP_065123

• Uniprot Accession Number:

  Q9GZV4

• Species:

  Human

• AA Sequence:

  MADEIDFTTG DAGASSTYPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDYQ LICIQDGYLS LLTETGEVRE DLKLPEGELG KEIEGKYNAG EDVQVSVMCA MSEEYAVAIK PCK