Products for Research Use Only
RegisterLogin

Human eIF-2 alpha/EIF2S1 antibody

CAT: 0112-ATGA0208-050Size: 50 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0112-ATGA0208-050Size:50 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Eukaryotic translation initiation factor 2, subunit 1 alpha (EIF2S1) is plays a central role in initiating translation, and provides for a rate-limiting step in protein synthesis. The phosphorylation of alpha-subunit of EIF2S1 effectively prevents formation of the EIF2S1/GTP/Met-tRNAi complex and inhibits global protein synthesis.
Product Name Alternative
Eukaryotic translation initiation factor 2 subunit alpha, EIF2, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, EIF-2alpha, EIF2A
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human EIF2S1 (1-315aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT5E10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
EIF2S1, ATGA0208-10 µg, ATGA0208-20 µg, ATGA0208-50 µg, ATGA0208-100 µg, ATGA0208-250 µg, ATGA0208-500 µg, ATGA0208-1 mg, ATGA0208-10, ATGA0208-20, ATGA0208-50, ATGA0208-100, ATGA0208-250, ATGA0208-500, ATGA0208-1
References & Citations
Kim JH, et al. (2011) . EMBO J, 1030 (12) :2454-64.; ; Pena J, et al. (2011) . J Biol Chem, 286 (16) :14226-36.; ; Muaddi H, et al. (2010) . Mol Biol Cell, 21 (18) :3220-31.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_004085
Uniprot Accession Number
P05198
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: Jurkat cell lysate ; ; Lane 3.: NIH-3T3 cell lysate
IF Description
ICC/IF analysis of EIF2S1 in HeLa cells. The cell was stained with ATGA0208 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

Related Products

CatalogName
ATGP0581-010Recombinant human eIF-2 alpha/EIF2S1 protein
ATGP0581-050Recombinant human eIF-2 alpha/EIF2S1 protein
ATGP0581-250Recombinant human eIF-2 alpha/EIF2S1 protein
ATGP1024-050Recombinant human eIF-2B alpha/EIF2B1 protein
ATGP1024-010Recombinant human eIF-2B alpha/EIF2B1 protein
ATGP1272-250Recombinant human eIF-3 alpha/EIF3J protein
ATGP1272-050Recombinant human eIF-3 alpha/EIF3J protein
ATGP1272-010Recombinant human eIF-3 alpha/EIF3J protein
ATGP0461-500Recombinant human eIF-1A/EIF1 protein
ATGP0461-020Recombinant human eIF-1A/EIF1 protein

Alternative Products

CatalogName
MBS4381845-01EIF2S1/EIF-2 alpha (EIF2A) Mouse Monoclonal Antibody [Clone PCRP-EIF2S1-1E2]
MBS4381844-01EIF2S1/EIF-2 alpha (EIF2A) Mouse Monoclonal Antibody [Clone PCRP-EIF2S1-1C11]
M04387Anti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298
E2237-02EIF2S1, aa1-315, Recombinant, Human (Eukaryotic Translation Initiation Factor 2 Subunit 1, Eukaryotic Translation Initiation Factor 2 Subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2A)
STJ92872Anti-eIF alpha antibody
abx404939-01EIF-2-Alpha Kinase GCN2 (EIF2AK4) Antibody
BP2169-500ugRecombinant Human eIF-2-alpha kinase activator GCN1
GTR18990340-01Human eIF-2-alpha kinase activator GCN1 Protein
abx659120-01Human Eif-2-Alpha Kinase Gcn2 (EIF2AK4) Protein
A01172Anti-eIF-2-alpha kinase GCN2 EIF2AK4 Antibody

Popular Products