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Human OSTF1 antibody

CAT: 0112-ATGA0125-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0125-050Size:50 µL
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Description
Osteoclast-stimulating factor (OSTF1) is an intracellular signaling protein, highly expressed by osteoclasts (OCL) . Indirectly, it enhances osteoclast (OCL) formation and bone resorption through a cellular signal transduction cascade by possibly interacting with c-Src or other c-Src-related proteins. OSTF1 contains a short proline-rich N-terminal region, an SH3 domain, three ankyrin repeats and an aspartate-rich C-terminal region, suggesting that it is potentially involved in protein-protein interactions.
Product Name Alternative
Osteoclast stimulating factor 1, SH3P2, OSF, Osteoclast stimulating factor 1
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human OSTF1 (1-217aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT9G4
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
OSTF1, ATGA0125-10 µg, ATGA0125-20 µg, ATGA0125-50 µg, ATGA0125-100 µg, ATGA0125-250 µg, ATGA0125-500 µg, ATGA0125-1 mg, ATGA0125-10, ATGA0125-20, ATGA0125-50, ATGA0125-100, ATGA0125-250, ATGA0125-500, ATGA0125-1
References & Citations
Kurihara N., et al. (2001) J. Biol. Chem. 276 (44) : 41035-9. ; ; Reddy S., et al. (1999) J Cell Physiol. 177 (4) : 636-45.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
AAH07459
Uniprot Accession Number
Q92882
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: MCF7 cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: LnCap cell lysate The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: OSTF1 Transfected 293T cell lysate {ATGA0125-WB2.jpg}
FACS Description
Flow cytometry analysis of OSTF1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of OSTF1 in MCF7 cells. The cell was stained with ATGA0125 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of OSTF1 in A431 cells. The cell was stained with ATGA0125 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0125-IF2.jpg}

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