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Human Synuclein-alpha/beta antibody

CAT: 0112-ASC0632-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ASC0632-100Size:100 µL
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Description
Alpha-synuclein (amino acids 119-140), an acidic neuronal protein of 140 amino acids, is extremely heat-resistant and is natively unfolded with an extended structure primarily composed of random coils. alpha-synuclein has been suggested to be implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders, and more recently, to be an important regulatory component of vesicular transport in neuronal cells. Specially, the non-Abeta component of amyloid plaques is a highly amyloidogenic peptide consisting of 35 amino acids which was first identified associated with senile plaques in the Alzheimer's disease brain.
Product Name Alternative
SNCA, NACP, PARK1, PARK4, PD1, α-synuclein Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease 4, autosomal dominant Lewy body
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human a-synuclein (119-140aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
3B6
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Synuclein beta, Synuclein alpha, SNCB protein, SNCB, SNCA protein, SNCA, PD1, Parkinson disease familial 1, PARK4, PARK1, Non A4 component of amyloid precursor, Non A beta component of AD amyloid, NACP, beta synuclein, alpha synuclein, a/b-Synuclein, a, b-Synuclein, a b-Synuclein, ASC0632-10 µg, ASC0632-20 µg, ASC0632-50 µg, ASC0632-100 µg, ASC0632-250 µg, ASC0632-500 µg, ASC0632-1 mg, ASC0632-010, ASC0632-020, ASC0632-050, ASC0632-100, ASC0632-250, ASC0632-500, ASC0632-01M
References & Citations
Jakes R., et al. (1994) FEBS lett. 345, 27-32.; ; Ueda K., et al. (1993) Proc. Natl.Acad. Sci. USA 90, 11282-11286.; ; Kim J. (1997) Molecules and Cells 7, 78-83.; ; Paik S. R., et al. (1997) Arch. Biochem. Biophys. 344, 325-334.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Neuroscience
NCBI Accession Number
NP_000336, NP_003076
Uniprot Accession Number
P37840, Q16143
WB Description
The recombinant protein (20ng) and tissue lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP60 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human alpha-synuclein protein; ; Lane 2.: Recombinant human beta-synuclein protein; ; Lane 3.: Recombinant human gamma-synuclein protein; ; Lane 4.: Mouse brain Tissue lysate; ; Lane 5.: Rat brain Tissue lysate;
FACS Description
Flow cytometry analysis of alpha, beta-Synuclein in LNCaP cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . Flow cytometry analysis of alpha, beta-Synuclein in U87MG cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {ASC0632-FC2.jpg}Flow cytometry analysis of alpha, beta-Synuclein in C6 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {ASC0632-FC3.jpg}
IF Description
ICC/IF analysis of alpha, beta-Synuclein in LNCaP cells. The cell was stained with ACS0632 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . ICC/IF analysis of alpha, beta-Synuclein in U87MG cells. The cell was stained with ACS0632 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ASC0632-IF2.jpg}ICC/IF analysis of alpha, beta-Synuclein in C6 cells. The cell was stained with ACS0632 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ASC0632-IF3.jpg}

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