Human alpha-Synuclein antibody

• Description:

  Alpha-synuclein (amino acids 1-140), an acidic neuronal protein of 140 amino acids, is extremely heat-resistant and is natively unfolded with an extended structure primarily composed of random coils. alpha-synuclein has been suggested to be implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders, and more recently, to be an important regulatory component of vesicular transport in neuronal cells. Specially, the non-Abeta component of amyloid plaques (NAC, amino acids 61-95) is a highly amyloidogenic peptide consisting of 35 amino acids which was first identified associated with senile plaques in the Alzheimer's disease brain.

• Product Name Alternative:

  SNCA, NACP, PARK1, PARK4, PD1, α-synuclein Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease 4, autosomal dominant Lewy body

• Host:

  Mouse

• Antigen Species:

  Human

• Reactivity:

  Human

• Immunogen:

  Recombinant human alpha-synuclein (61-95) purified from E. coli

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  5C2

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB, ICC/IF

• Purification Method:

  By protein-A affinity chromatography

• Concentration:

  1 mg/mL (determined by BCA assay)

• Additionnal Information:

  A-Synuclein, ANC0603-10 µg, ANC0603-20 µg, ANC0603-50 µg, ANC0603-100 µg, ANC0603-250 µg, ANC0603-500 µg, ANC0603-1 mg, ANC0603-10, ANC0603-20, ANC0603-50, ANC0603-100, ANC0603-250, ANC0603-500, ANC0603-1

• References & Citations:

  Jakes, R., et al. (1994) FEBS lett. 345:27-32.; ; Ueda, K., et al. (1993) Proc. Natl.Acad. Sci. USA 90:11282-11286.; ; Kim, J. (1997) Molecules and Cells 7:78-83.; ; Paik, S. R., et al. (1997) Arch. Biochem. Biophys. 344:325-334.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes:

  The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category:

  Neuroscience

• NCBI Accession Number:

  NP_000336

• Uniprot Accession Number:

  P37840

• WB Description:

  The recombinant proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human alpha-synuclein protein; ; Lane 2.: Recombinant human beta-synuclein protein; ; Lane 3.: Recombinant human gamma-synuclein proteinThe recombinant human alpha-synuclein domains were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein (61-95aa) antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human a-synuclein (1-60aa) ; ; Lane 2.: Recombinant human a-synuclein (1-95aa) ; ; Lane 3.: Recombinant human a-synuclein (61-140aa) ; ; Lane 4.: Recombinant human a-synuclein (96-140aa) {2004010016919061.jpg}

• IF Description:

  ICC/IF analysis of alpha-Synuclein in HeLa cells. The cell was stained with ANC0603 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .