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Human PDCD12 antibody

CAT: 0112-APD0912-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-APD0912-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PDCD12 (Programmed cell death 12), also referred to as AVEN, represents a new class of cell death regulator. PDCD12 is a conserved intracellular membrane protein that blocks apoptosis impeded by Apaf-1 mediated caspase activation. Bcl-2 and BAD also interact with PDCD12 to prevent apoptosis. It is expressed in a wide variety of normal tissues including heart, skeletal muscle, kidney, liver, testis, and diseases such as cancer
Product Name Alternative
Cell death regulator aven, apoptosis, caspase activation inhibitor, PDCD12, programmed cell death 12
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PDCD12 (254-362aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
3G4
Conjugation
Unconjugated
Applications
ELISA, WB, IHC
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PDCD12, APD0912-10 µg, APD0912-20 µg, APD0912-50 µg, APD0912-100 µg, APD0912-250 µg, APD0912-500 µg, APD0912-1 mg, APD0912-10, APD0912-20, APD0912-50, APD0912-100, APD0912-250, APD0912-500, APD0912-1
References & Citations
Figueroa B Jr, et al: (2004) Biotechnol Bioeng. 85 (6), 589-600.; ; Cheng EH, et al: (2000) Mol Cell. 6 (1), 31-40.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
NCBI Accession Number
NP_065104
Uniprot Accession Number
Q9NQS1
WB Description
The tissue lysate of mouse kidney (50 µg) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD12 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
IHC description
Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

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