Human PDCD4 antibody

• Description:

  Programmed cell death 4 (PDCD4) encodes a tumor suppressor protein whose expression is lost in progressed carcinomas of lung, breast, colon, and prostate. The expression of PDCD4 gene is strongly induced during apoptosis in a number of cell types. It is modulated by cytokines in natural killer and T cells.

• Product Name Alternative:

  Programmed cell death 4 isoform 1, neoplastic transformation inhibitor, H731, MGC33046, MGC33047, Programmed cell death 4 isoform 1, PDPC4, Programmed cell death 4 isoform 1, PDCD4, programmed cell death 4 (neoplastic transformation inhibitor), nuclear antigen H731

• Host:

  Mouse

• Antigen Species:

  Human

• Reactivity:

  Human

• Immunogen:

  Recombinant human PDCD4 (1-469aa) purified from E. coli

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  4C2

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB, ICC/IF, FACS

• Purification Method:

  By protein-A affinity chromatography

• Concentration:

  1 mg/mL (determined by BCA assay)

• Additionnal Information:

  ATGA0601-10 µg, ATGA0601-20 µg, ATGA0601-50 µg, ATGA0601-100 µg, ATGA0601-250 µg, ATGA0601-500 µg, ATGA0601-1 mg, ATGA0601-10, ATGA0601-20, ATGA0601-50, ATGA0601-100, ATGA0601-250, ATGA0601-500, ATGA0601-1

• References & Citations:

  Palamarchuk A, et al., (2006) Cancer Res 65 (24) :11282-11286.; ; Yoshina, et al., (1999) Pathol Int 49 (12) :1067-1077.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes:

  The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category:

  Cancer, Epigenetics and Nuclear Signaling

• NCBI Accession Number:

  NP_055271

• Uniprot Accession Number:

  Q53EL6

• WB Description:

  The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD4 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: K562 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: 293T cell lysate

• FACS Description:

  Flow cytometry analysis of PDCD4 in MCF7 cells. The cell was stained with ATGA0601 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

• IF Description:

  ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with ATGA0601 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .