Human PDCD4 antibody

CAT: 0112-APD0818-050Size: 50 µLDry Ice: NoHazardous: No
CAT#:0112-APD0818-050Size:50 µL
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Description
Programmed cell death 4 (PDCD4) encodes a tumor suppressor protein whose expression is lost in progressed carcinomas of lung, breast, colon, and prostate. The expression of PDCD4 gene is strongly induced during apoptosis in a number of cell types. It is modulated by cytokines in natural killer and T cells.
Product Name Alternative
Programmed cell death 4 isoform 1, neoplastic transformation inhibitor, H731, MGC33046, MGC33047, Programmed cell death 4 isoform 1, PDPC4, Programmed cell death 4 isoform 1, PDCD4, programmed cell death 4 (neoplastic transformation inhibitor), nuclear antigen H731
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PDCD4 (1-469aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
K4C1
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PDCD4, Programmed cell death 4 isoform 1, neoplastic transformation inhibitor, H731, MGC33046, MGC33047, Programmed cell death 4 isoform 1, PDPC4, Programmed cell death 4 isoform 1, PDCD4, programmed cell death 4 (neoplastic transformation inhibitor), nuclear antigen H731, APD0818-10 µg, APD0818-20 µg, APD0818-50 µg, APD0818-100 µg, APD0818-250 µg, APD0818-500 µg, APD0818-1 mg, APD0818-10, APD0818-20, APD0818-50, APD0818-100, APD0818-250, APD0818-500, APD0818-1
References & Citations
Palamarchuk A, et al., (2006) Cancer Res 65 (24) :11282-11286.; ; Yoshina, et al., (1999) Pathol Int 49 (12) :1067-1077.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
NCBI Accession Number
NP_055271
Uniprot Accession Number
Q53EL6
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD4 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: K562 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: 293T cell lysate;
FACS Description
Flow cytometry analysis of PDCD4 in MCF7 cells. The cell was stained with APD0818 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with APD0818 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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