Products for Research Use Only
RegisterLogin

Human HSP90 alpha antibody

CAT: 0112-AHS0704-050Size: 50 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0112-AHS0704-050Size:50 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Hsp90 is a human heat shock protein. In response to adverse change in their environment, cell from all organisms increase the expression of a class of proteins referred to as heat shock or stress protein. The Hsp90, a highly conserved stress-induced protein, is abundantly expressed in most tissues under non-stress conditions and is required for eukaryotic cell viability. Hsp90 is primarily a cytoplasmic protein and its function remains unknown. It exists in a dimeric form and has been observed to bind to several other cellular proteins such as retro-virus kinases, steroid receptor, heme-regulated protein kinase, actin and tubulin.
Product Name Alternative
Heat shock protein HSP 90-alpha, HSP 86, Renal carcinoma antigen NY-REN-38, HSPC1, HSPCA, Heat shock protein 90-alpha Renal carcinoma antigen NY REN 38, D6S182, FLJ26984, FLJ31884, Heat shock 90kDa protein 1 alpha, heat shock protein 90kDa alpha (cytosolic), class A member 2, HSP84, HSP86, Hsp89, HSP89A, Hsp90, HSP90A, HSP90AA1, HSP90ALPHA, HSP90N, HSPCAL3, HSPCB, HSPN, LAP2, Lipopolysaccharide associated protein2, LPS associated protein 2, NY REN 38 antigen, hsp90, Heat shock protein HSP 90-alpha
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human Hsp90 (1-732aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
4F10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, IHC, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
HSP90 a, AHS0704-10 µg, AHS0704-20 µg, AHS0704-50 µg, AHS0704-100 µg, AHS0704-250 µg, AHS0704-500 µg, AHS0704-1 mg, AHS0704-10, AHS0704-20, AHS0704-50, AHS0704-100, AHS0704-250, AHS0704-500, AHS0704-1
References & Citations
B-T, et al., (1984) Molecular & Cellular Biol 4: 2802-2810.; ; Pritchard, K. A, et al., (2001) J Biol Chem 276:17621-17624.; ; Miyamoto, A, et al., (2002) Nature 416:865-869.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_005339
Uniprot Accession Number
P07900
WB Description
The cell lysates (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate;
IHC description
Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
FACS Description
Flow cytometry analysis of HSP90 in HepG2 cells. The cell was stained with AHS0704 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of HSP90 in HeLa cells. The cell was stained with AHS00704 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

Related Products

CatalogName
HSP0501-100Recombinant human HSP90 alpha protein
HSP0501-500Recombinant human HSP90 alpha protein
HSP0501-020Recombinant human HSP90 alpha protein
ATGA0488-050Human alpha-FetoproteiFP antibody
ATGA0466-050Human PGC1 alpha antibody
ATGA0244-050Human alpha-Synuclein antibody
ATGA0488-100Human alpha-FetoproteiFP antibody
ATGA0244-100Human alpha-Synuclein antibody
ANC0603-100Human alpha-Synuclein antibody
ATGA0466-100Human PGC1 alpha antibody

Alternative Products

CatalogName
GTR18894554-01Human Hsp90 alpha
OM304672-01HSP90 Alpha Human
DF6101-01Hsp90 alpha Antibody
KC-6262-01Hsp90-alpha Antibody

Popular Products