Leptin Rat, PEG

CAT: 1071-OM301101-01Size: 20 µgDry Ice: NoHazardous: No

CAT#:1071-OM301101-01Size:20 µg

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AVAILABILITY: InStock

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Mono-Pegylated Leptin Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and an additional Ala at N-terminus having a molecular mass of 35.6 kDa (with 20 kDa PEG) as determined by mass spectometry. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 48 kDa protein and in gel-filtration on Superdex 200 as over 100 kDa protein. Its half-life in circulation after SC injection was over 20 hours. Rat Leptin was purified by proprietary chromatographic techniques according to Salomon et al (2006) Protein Expression and Purification 47, 128–136 and then pegylated.

Swiss Prot

P50596

Source

Escherichia Coli.

Buffer

The protein was lyophilized from a concentrated (0.65 mg/mL) solution with 03mM NaHCO3.

Storage Conditions

Lyophilized Rat Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.

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