Leptin tA Rat

• Description:

  Leptin Antagonist Triple Mutant Rat Recombinant is a singly non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A mutant. Leptin Antagonist Triple Mutant Rat Recombinant was purified by proprietary chromatographic techniques.

• Source:

  Escherichia coli.

• Buffer:

  The protein was lyophilized from a concentrated (0.85 mg/mL) solution with 03mM NaHCO3.

• Storage Conditions:

  Lyophilized Leptin-Antagonist Triple Mutant Rat Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin mutant mg/mL and up to 2 mM and filter sterilization LEP-tA mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested.

• Sequence Similarities:

  The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.