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HCM005 EC Medium (EC Broth)

CAT: 0938-HCM005Size: 500 g/bottleDry Ice: NoHazardous: No
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CAT#:0938-HCM005Size:500 g/bottle
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Assay Principle
Tryptone provide carbon and nitrogen sources; the 3rd bile suppress gram-positive bacteria, especially against Gram-positive bacteria and fecal streptococci; lactose is fermentable sugars; potassium hydrogen phosphate and potassium dihydrogen phosphate as buffer ; sodium chloride maintains osmotic equilibrium. Formulation (per liter) : Tryptone: 20.0g The 3rd bile: 1.5g Lactose: 5.0g Dipotassium hydrogen: 4.0g Potassium dihydrogen phosphate: 1.5g Sodium chloride:5.0g Final pH 6.9 ± 0.2
Assay Protocol
1.Suspend 37g in 1 L of distilled water or deionized water, stirring heated to boiling until completely dissolved, packed in with a small test tube inverted tube, and autoclave at 121 °C for 15min, leave to cool to room temperature and set aside. 2.Diluted and treated sample.
Storage Conditions
Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Recommended Usage
For determination of fecal coliforms and E. coli confirmatory testing .

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