HCM017 Macconkey Agar

Peptones are sources of nitrogen and other nutrients. Lactose is a fermentable carbohydrate. When lactose is fermented, alocal pH drop around the colony causes a color change in the pH indicator (neutral red) and bile precipitation. Bile salts, bile salts no. 3, oxgall and crystal violet are selective agents that inhibit growth of gram-positive organisms. Agar is the solidifying agent. Formulation (per liter) : Pancreatic Digest of Gelatin 17.0 g Peptones (meat and casein) 3.0 g Lactose Monohydrate 10.0 g Sodium Chloride 5.0 g Bile Salts 1.5 g Agar 13.5 g Neutral Red 30.0 mg Crystal Violet 1 mg Final pH 7.1±0.2 How to use: 1.Suspend 50 g in 1 L of distilled or deionized water. Heat to boiling to dissolve completely. Autoclave at 121°C for 15 minutes. 2.transfer 1 mL of Soybean–Casein Digest Broth to 100 mL of MacConkey Broth, and incubate at 42 to 44 for 24 to 48 hours. Subculture on a plate of MacConkey Agar at 30 to 35 deg.C for 18 to 72 hours. Quality control: Item The name and number of strain PR/G Reaction Growth rate E.Coli ATCC8739 PR≥0.7 Rose-red Characteristic difference Proteus mirabilis CMCC (b) 49005 PR≥0.7 Colorless, no swarming Selective Staphylococcus aureus ATCC6538 G≤1 -

Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of three years.