ORC1 Antibody / Origin Recognition Complex 1

ORC1 Antibody / Origin Recognition Complex 1

• Description: The initiation of DNA replication is a multi-step process that depends on the formation of pre-replication complexes, which trigger initiation. Among the proteins required for establishing these complexes are the origin recognition complex (ORC) proteins. ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors. Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis. ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle; however, only ORC2 remains stably bound to chromatin. ORC4 and ORC6 are also expressed constantly throughout the cell cycle. ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble. The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells.
• CAS Number: 9007-83-4
• UniProt: Q13415
• Host: Mouse
• Immunogen: A recombinant full-length human ORC1 protein was used as the immunogen for this ORC1 antibody.
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Applications: ELISA
• Format: Purified
• Buffer: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Reconstitution: Store the ORC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
• Limitations: This ORC1 antibody is available for research use only.