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HDAC3 Antibody

CAT: 0800-R30679Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30679Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Histone deacetylase 3 is a member of the histone deacetylase/acuc/apha family of proteins and is an enzyme that in humans is encoded by the HDAC3 gene. The enzyme has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. The protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. And this gene is regarded as a potential tumor suppressor gene. HDAC3 has an open reading frame of 428 amino acids and shares 53% amino acid identity with HDAC1 and 52% with HDAC2. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. All experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 resulted in loss of enzymatic activity associated with HDAC4.
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL
UniProt
O15379
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 411-428 (NEFYDGDHDNDKESDVEI) were used as the immunogen for this HDAC3 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F, ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Limitations
This HDAC3 antibody is available for research use only.
Storage Conditions
After reconstitution, the HDAC3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the HDAC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear, cytoplasmic
Image Legend
Western blot testing of HDAC3 antibody and Lane 1: rat stomach; 2: rat testis; 3: MCF-7; 4: HeLa; 5: Jurkat; 6: SKOV cell lysate. Predicted molecular weight ~49 kDa.

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