Products for Research Use Only

ARAF Antibody / A-Raf

CAT: 0800-RQ4970Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ4970Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Serine/threonine-protein kinase A-Raf or simply A-Raf is an enzyme that in humans is encoded by the ARAF gene. It is mapped to Xp11.3. This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases.
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P10398
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids E309-T413 from the human protein were used as the immunogen for the ARAF antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Limitations
This ARAF antibody is available for research use only.
Storage Conditions
Store the ARAF antibody at -20°C.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ARAF antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human MCF7 cells with ARAF antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · P10398

Serine/threonine-protein kinase A-Raf

ARAF_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P10398
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ARAF
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.11.1
Processing
—
Secondary accessions
P07557, Q5H9B2, Q5H9B3
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingMetal-bindingNucleotide-bindingZinc

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

PTM

Phosphoprotein

Disease

Proto-oncogene

Domain

Zinc-finger