Dnmt3a Antibody

CAT: 0800-V3827-20UGSize: 20 µgDry Ice: NoHazardous: No
CAT#:0800-V3827-20UGSize:20 µg
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Description
DNMT3A is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western Blot: 1-2 µg/mL
UniProt
Q16655
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full length human protein was used as the immunogen for this Dnmt3a antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
PCRP-DNMT3A-1E2
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Limitations
This Dnmt3a antibody is available for research use only.
Storage Conditions
Store the Dnmt3a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Dnmt3a antibody to be titered up or down for optimal performance.
Location
Nuclear
Image Legend
IHC testing of FFPE human placenta with Dnmt3a antibody. Required HIER: boil sections in 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.

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