Dnmt3a Antibody

• Description:

  DNMT3A is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western Blot: 1-2 µg/mL

• UniProt:

  Q16655

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full length human protein was used as the immunogen for this Dnmt3a antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  PCRP-DNMT3A-1E2

• Applications:

  IHC-P, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Limitations:

  This Dnmt3a antibody is available for research use only.

• Storage Conditions:

  Store the Dnmt3a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Dnmt3a antibody to be titered up or down for optimal performance.

• Location:

  Nuclear

• Image Legend:

  IHC testing of FFPE human placenta with Dnmt3a antibody. Required HIER: boil sections in 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.