Recombinant Human NEDD8

• Background:

  Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.

• Product Name Alternative:

  Neural precursor cell expressed developmentally down-regulated protein 8; NEDD8; Neddylin; Ubiquitin-like protein Nedd8

• Accession Number:

  Q15843

• Host:

  E.coli

• Endotoxin:

  <1 EU/µg as determined by LAL test.

• Purity:

  Greater than 95% as determined by reducing SDS-PAGE

• Form:

  Lyophilized

• Reconstitution:

  Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

• Molecular Weight:

  8.6 KDa

• Shipping Conditions:

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions:

  Store at -70°C/-20°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, pH 8.0.

• Construct:

  Recombinant Human Neural precursor cell expressed developmentally down-regulated protein 8 is produced by our E.coli expression system and the target gene encoding Met1-Gly76 is expressed.

• Species:

  Human