PEK/PERK (Phospho-Thr982) Antibody

• Background:

  Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR) -induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) .

• Description:

  This is a PEK/PERK (Phospho-Thr982) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  E2AK3; PERK; PEK; EIF2AK3

• Gene ID:

  9451

• Swiss Prot:

  Q9NZJ5

• Reactivity:

  Human

• Immunogen:

  Peptide sequence around phosphorylation site of threonine 982 (R-H-T (p) -G-Q) derived from Human PEK/PERK.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  IHC

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  IHC 1:50-1:100

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Observed Molecular Weight:

  125kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG