MEK2 (Phospho-Thr394) Antibody

• Background:

  Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.

• Description:

  This is a MEK2 (Phospho-Thr394) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  ERK activator kinase 2; MAP kinase kinase 2; MAP2K2; MAPK/ERK kinase 2; MAPKK 2

• Gene ID:

  5605

• Swiss Prot:

  P36507

• Reactivity:

  Human

• Immunogen:

  Peptide sequence around phosphorylation site of threonine 394 (P-G-T (p) -P-T) derived from Human MEK-2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC, IF

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IHC 1:50-1:100, IF 1:100-1:200

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Host or Source:

  Rabbit

• Isotype:

  IgG