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Β-NGF / Beta-NGF Protein

CAT: 0209-M60-134-L1000Size: 1000 µgDry Ice: YesHazardous: No
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CAT#:0209-M60-134-L1000Size:1000 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.
Synonyms
Beta-NGF Protein; Ngf Protein; Ngfb Protein
Accession Number
NP_001106168.1
Reactivity
Mouse
Cross Reactivity
Mouse
Assay Protocol
See Datasheet send with the product
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-6 ng/mL.
Length
121
Form
Lyophilized or frozen liquid
Buffer
150mM NaCl, 50mM NaAc, pH 5.5.
Reconstitution
See Datasheet
Molecular Weight
14 kDa
Storage Conditions
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Host or Source
CHO cells

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