NGF-beta

• Description:

  Β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of murine β-NGF is a noncovalently disulfide-linked homodimer, of two 13.4 kDa polypeptide monomers (240 total amino acid residues) . The three disulfide bonds are required for biological activity.

• Synonyms:

  Ngf; Ngfb

• NCBI Gene ID:

  18049

• UniProt:

  P01139

• Accession Number:

  NP_038637.1

• Accession Number mRNA:

  NM_013609

• Chromosomal Location:

  3 F2.2; 3 48.5 cM

• Reactivity:

  Mouse

• Cross Reactivity:

  Human, Mouse

• Sequence:

  SSTHPVFHMG EFSVCDSVSV WVGDKTTATD IKGKEVTVLA EVNINNSVFR QYFFETKCRA SNPVESGCRG IDSKHWNSYC TTTHTFVKAL TTDEKQAAWR FIRIDTACVC VLSRKATRRG

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analysis

• Bioactivity:

  The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

• Length:

  240

• Form:

  Lyophilized

• Molecular Weight:

  13.4 kDa

• Host or Source:

  E. coli