Β-NGF / Beta-NGF Protein

• Description:

  Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

• Synonyms:

  Beta-NGF Protein; HSAN5 Protein; NGFB Protein

• Accession Number:

  NP_002497.2

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  ≥ 95 % as determined by SDS-PAGE, ≥ 90 % as determined by SEC-HPLC, ≥ 90% as determined by SEC-MALS

• Bioactivity:

  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/mL.

• Length:

  118

• Form:

  Lyophilized

• Buffer:

  40mM His, 40mM Arg, 150mM NaCl, pH 5.5

• Reconstitution:

  See Datasheet

• Molecular Weight:

  30 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  CHO cells