TRAIL

• Description:

  TRAIL is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain”, which activates the cell’s apoptotic machinery. Recombinant murine TRAIL is a 174 amino acid polypeptide (20.0 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL protein.

• Synonyms:

  Tnfsf10; TL2; Ly81; Trail; APO-2L; AI448571; A330042I21Rik

• NCBI Gene ID:

  22035

• UniProt:

  P50592

• Accession Number:

  NP_033451.1

• Accession Number mRNA:

  NM_009425

• Chromosomal Location:

  3 A3; 3

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Sequence:

  MRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 95% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Assay#1: Determined by the dose-dependent stimulation of MIP-2 production by mouse spleen cells using a concentration range of 10-100 ng/ml. Assay#2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells) . The expected ED50 for this effect is 40.0-60.0 ng/ml.

• Length:

  174

• Form:

  Lyophilized

• Molecular Weight:

  20 kDa

• Host or Source:

  E. coli