PDGF-BB

• Description :

  PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-BB is a 24.4 kDa disulfide-linked homodimer of two B chains (218 total amino acids) .

• Synonyms :

  Platelet-derived growth factor-BB

• NCBI Gene ID :

  18591

• UniProt :

  P31240

• Accession Number :

  NP_035187.2

• Accession Number mRNA :

  NM_011057.3

• Reactivity :

  Mouse

• Cross Reactivity :

  Mouse, Monkey, Human

• Sequence :

  SLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity :

  Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 ng/ml.

• Length :

  218

• Form :

  Lyophilized

• Molecular Weight :

  24.4 kDa

• Host or Source :

  E. coli