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PDGF-AA

CAT: 0209-200-052Size: 20 µgDry Ice: NoHazardous: No
CAT#:0209-200-052Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids) .
Synonyms
PDGFA; PDGF1; PDGF-A
NCBI Gene ID
5154
UniProt
P04085
Accession Number
NP_002598.5
Accession Number mRNA
NM_002607.5
Chromosomal Location
7p22
Reactivity
Human
Cross Reactivity
Human
Sequence
SIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT
Assay Protocol
Centrifuge vial prior to opening. The lyophilized PDGF-AA should be reconstituted in water to a concentration not lower than 50 µg/ml. For long term storage we recommend to add at least 0.1% human or bovine serum albumin.
Endotoxin
< 0.1 ng per ug of PDGF-AA
Purity
> 95% by SDS-PAGE
Bioactivity
The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts) .
Length
125
Form
Lyophilized
Buffer
50mM acetic acid
Reconstitution
50mM acetic acid
Molecular Weight
28.5 kDa
Storage Conditions
The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AA is best stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles.
Host or Source
E. coli
N Terminal Sequence
SIEEAVPA

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