PDGF-AA

• Description:

  PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids) .

• Synonyms:

  PDGFA; PDGF1; PDGF-A

• NCBI Gene ID:

  5154

• UniProt:

  P04085

• Accession Number:

  NP_002598.5

• Accession Number mRNA:

  NM_002607.5

• Chromosomal Location:

  7p22

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  SIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT

• Assay Protocol:

  Centrifuge vial prior to opening. The lyophilized PDGF-AA should be reconstituted in water to a concentration not lower than 50 µg/ml. For long term storage we recommend to add at least 0.1% human or bovine serum albumin.

• Endotoxin:

  < 0.1 ng per ug of PDGF-AA

• Purity:

  > 95% by SDS-PAGE

• Bioactivity:

  The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts) .

• Length:

  125

• Form:

  Lyophilized

• Buffer:

  50mM acetic acid

• Reconstitution:

  50mM acetic acid

• Molecular Weight:

  28.5 kDa

• Storage Conditions:

  The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AA is best stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles.

• Host or Source:

  E. coli

• N Terminal Sequence:

  SIEEAVPA