IL-35

• Description:

  Interleukin-35 is a glycosylated, heterodimeric protein consisting of the p35 subunit from IL-12 (IL-12α) and the β subunit from IL-27 (EBI3) . IL-35 can be expressed by regulatory T-cells (Tregs), macrophages, and certain trophoblast and dendritic cells. It is induced in response to inflammation, and generally acts as an inflammation suppressor. IL-35 suppresses inflammation by exerting multiple activities, including the induction of regulatory T-cells and the suppression of Th17 cells. Recombinant Human IL-35 produced from HEK293 cells is a glycosylated protein, primarily heterodimeric, that migrates as a diffuse band centered at an apparent molecular weight of about 60 kDa by SDS-PAGE analysis under non-reducing conditions. Recombinant Human IL-35 expressed in HEK293 cells contains 406 amino acid residues and has a calculated molecular weight of 45.8 kDa.

• Synonyms:

  Interleukin-35

• NCBI Gene ID:

  Alpha: 3592 Beta: 10148

• UniProt:

  Alpha: P29459 Beta: Q14213

• Accession Number:

  Alpha: NP_001384921 Beta: NP_005746

• Accession Number mRNA:

  Alpha: NM_001397992 Beta: NM_005755

• Reactivity:

  Human

• Cross Reactivity:

  Human, Mouse

• Sequence:

  Α Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS β Subunit: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  ≥ 90% by SDS-PAGE gel and HPLC analyses.

• Bioactivity:

  Measured by its ability to inhibit proliferative activity of PBMCs.

• Length:

  406

• Form:

  Lyophilized

• Molecular Weight:

  45.8 kDa

• Host or Source:

  HEK 293 cells