IL-18 (IL-1F4)

• Description:

  Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δT cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases. IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation. IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs.

• Synonyms:

  Interleukin-18, Iboctadekin, Interferon gamma-inducing factor, (IFN-gamma-inducing factor), Interleukin-1 gamma (IL-1 gamma)

• NCBI Gene ID:

  3606

• UniProt:

  Q14116

• Accession Number:

  NP_001230140.1

• Accession Number mRNA:

  NM_001243211.1

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human IL-18 (IL-1F4)

• Clone:

  (#42U51)

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG1