IL-21

• Description :

  IL‐21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen‐specific responses of immune cells. The biological effects of IL‐21 include: inducing the differentiation of T cell‐stimulated B cells into plasma cells and memory B cells; the stimulation of IgG production in conjunction with IL‐4; and the induction of apoptotic effects in naïve B cells and stimulated B cells in the absence of T cell signaling. Additionally, IL‐21 promotes the anti‐tumor activity of CD8+ T cells and NK cells. IL‐21 exerts its effect through binding to a specific type I cytokine receptor, IL‐21R, which also contains the γ chain (γc) found in other cytokine receptors, including IL‐2, IL‐4, IL‐7, IL‐9 and IL‐15. The IL‐21/IL‐21R interaction triggers a cascade of events, which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Recombinant Rat IL‐21 is a 15.3 kDa protein containing 130 amino acid residues.

• Synonyms :

  IL21, Interleukin-21

• NCBI Gene ID :

  365769

• UniProt :

  A3QPB9

• Accession Number :

  NP_001102413.1

• Accession Number mRNA :

  NM_001108943.2

• Reactivity :

  Rat

• Cross Reactivity :

  Rat

• Sequence :

  MHKSSPQRPD HLLIRLRHLM DIVEQLKIYE NDLDPELLTA PQDVKGQCEH EAFACFQKAK LKPSNTGNNK TFINDLLAQL RRRLPAKRTG NKQRHMAKCP SCDLYEKKTP KEFLERLKWL LQKMIHQHLS

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  ≥ 98% by SDS-PAGE gel and HPLC analyses.

• Bioactivity :

  Determined by its ability to stimulate the proliferation of human ANBL-6 cells. The expected ED50 is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106 units/mg.

• Length :

  130

• Form :

  Lyophilized

• Molecular Weight :

  15.3 kDa

• Host or Source :

  E. coli