IL-13 Variant

• Description :

  IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 is cross-species reactive. A variant of IL-13 shows enhanced functional activity compared with the wild type IL-13. The genetic variant, termed human IL-13 analog, is a mature 114 amino acid protein with a substitution of Q for R at position 112.

• Synonyms :

  IL13; ALRH; BHR1; P600; IL-13

• NCBI Gene ID :

  3596

• UniProt :

  P35225

• Accession Number :

  NP_002179.2

• Accession Number mRNA :

  NM_002188

• Chromosomal Location :

  5q31

• Reactivity :

  Human

• Cross Reactivity :

  Mouse, Human

• Sequence :

  MSPGPVPPST ALRELIEELV NITQNQKAPL CNGSMVWSIN LTAGMYCAAL ESLINVSGCS AIEKTQRMLS GFCPHKVSAG QFSSLHVRDT KIEVAQFVKD LLLHLKKLFR EGQFN

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity :

  This IL-13 analog shows a two fold increase, relative to wild type IL-13, in bioactivity as measured by the in-vitro dose dependent activation of STAT6 and IL-13 dependent gene induction in transfected A201.1 cells. This analog has also been shown to exhibit increased in vivo activity compared to wild type IL-13, as measured by the induction of airway hyper-responsiveness.

• Length :

  114

• Form :

  Lyophilized

• Molecular Weight :

  12.3 kDa

• Host or Source :

  E. coli