IL-13

• Description:

  IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 is cross-species reactive. Recombinant murine IL-13 is a 12.3 kDa protein consisting of 111 amino acid residues.

• Synonyms:

  Il13; Il-13

• NCBI Gene ID:

  16163

• UniProt:

  P20109

• Accession Number:

  NP_032381.1

• Accession Number mRNA:

  NM_008355

• Chromosomal Location:

  11 B1.3; 11 29.0 cM

• Reactivity:

  Mouse

• Cross Reactivity:

  Human, Mouse

• Sequence:

  MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The ED50 as determined by the dose-dependent proliferation of TF-1 cells was < 4.0 ng/ml, corresponding to a specific activity of > 2.5 x 105 units/mg.

• Length:

  111

• Form:

  Lyophilized

• Buffer:

  10 mM sodium citrate pH 4.0

• Molecular Weight:

  12.3 kDa

• Host or Source:

  E. coli