IFNG / Interferon Gamma Protein

• Description:

  IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and tumor control. Aberrant IFN gamma expression is associated with some autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.

• Synonyms:

  If2f Protein; Ifg Protein; IFN-g Protein; Ifng Protein

• Accession Number:

  NP_032363.1

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  > 95 % as determined by SDS-PAGE

• Bioactivity:

  1. Measured by its binding ability in a functional ELISA. Immobilized Mouse Interferon Gamma at 2 μg/mL (100 μL/well) can bind Mouse IFNGR1 hFc, the EC50 of Mouse IFNGR1 hFc is 7.0-30.0 ng/mL. 2. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV) . The ED50 for this effect is 0.05-0.3 ng/mL.

• Length:

  133

• Form:

  Lyophilized

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  19 kDa (glycosylated)

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells