IFN lambda1 (IL-29)

• Description:

  IFN lambda-1, -2, and -3 are distantly related to the IL-10 family and interferons. All three IFN-lambdas use a distinct receptor system composed of an IFN-lambdaR1 subunit (also called CRF2-12) and IL-10R2 subunit (also called CRF2-14) . Signaling through this receptor system induces antiviral defense similar but distinct than the type I interferons (Kotenko, SV. et al. Nat Immunology 2003 [1] p. 69-77) . Recombinant human IFN-lambda 1 is a 19.8 kDa protein containing 178 amino acid residues.

• Synonyms:

  IL29; IFNL1; IL-29; IL-29, IL-28A; IL-28B

• NCBI Gene ID:

  282618

• UniProt:

  Q8IU54

• Accession Number:

  NP_742152.1

• Accession Number mRNA:

  NM_172140.1

• Chromosomal Location:

  19q13.13

• Reactivity:

  Human

• Cross Reactivity:

  Human, Monkey

• Sequence:

  PTSKPTTTGK GCHIGRFKSL SPQELASFKK ARDALEESLK LKNWSCSSPV FPGNWDLRLL QVRERPVALE AELALTLKVL EAAAGPALED VLDQPLHTLH HILSQLQACI QPQPTAGPRP RGRLHHWLHR LQEAPKKESA GCLEASVTFN LFRLLTRDLK YVADGNLCLR TSTHPEST

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to activate STAT phosphorylation in an ISRE Luciferase Reporter Assay using human colon carcinoma COLO205 cells. The expected ED50 is 0.2-0.5 ng/ml.

• Length:

  178

• Form:

  Lyophilized

• Molecular Weight:

  19.8 kDa

• Host or Source:

  E. coli