GDF-2

• Description :

  GDF-2 belongs to the TGF-ß cytokine family whose members play an important role during prenatal development and postnatal growth, remodeling and maintenance of a variety of tissues and organs. GDF-2 is expressed mainly in nonparenchymal cells of the liver, but is also found in other various cells and tissues. GDF-2 can signal through the ALK1 receptor and has been implicated in a number of physiologic events. These include regulation of the hepatic reticuloendothelial system, glucose homeostasis, and iron homeostasis, as well as the inhibition of angiogenesis. Recombinant human GDF-2 is a 24.1 kDa disulfide linked homodimeric protein consisting of two 110 amino acid polypeptide chains.

• Synonyms :

  GDF2; BMP9; BMP-9

• NCBI Gene ID :

  2658

• UniProt :

  Q9UK05

• Accession Number :

  NP_057288

• Accession Number mRNA :

  NM_016204

• Chromosomal Location :

  10q11.22

• Reactivity :

  Human

• Cross Reactivity :

  Mouse

• Sequence :

  SAGAGSHCQK TSLRVNFEDI GWDSWIIAPK EYEAYECKGG CFFPLADDVT PTKHAIVQTL VHLKFPTKVG KACCVPTKLS PISVLYKDDM GVPTLKYHYE GMSVAECGCR

• Assay Protocol :

  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  > 95% by SDS-PAGE & HPLC analyses

• Bioactivity :

  Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.9 ng/ml.

• Length :

  110

• Form :

  Lyophilized

• Reconstitution :

  Water

• Molecular Weight :

  24.1 kDa

• Storage Conditions :

  The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

• Host or Source :

  CHO cells