GDF-11

• Description:

  GDF-11 is a myostatin-homologous protein that acts as an inhibitor of nerve tissue growth. GDF-11 has been shown to suppress neurogenesis through a myostatin-like pathway, which involves arrest of progenitor cell-cycle in the G1 phase. Similarities between myostatin and GDF-11, which are 90% identical in their amino acid sequence, suggests that the regulatory mechanisms responsible for maintaining proper tissue size during neural and muscular development might be the same. Recombinant human GDF-11 is a 25.0 kDa disulfide-linked homodimer containing two 109 amino acid polypeptide chains. It is highly homologous to myostatin/GDF-8 sharing 90% amino-acid sequence identity.

• Synonyms:

  GDF11; BMP11; BMP-11

• NCBI Gene ID:

  10220

• UniProt:

  O95390

• Accession Number:

  NP_005802

• Accession Number mRNA:

  NM_005811

• Chromosomal Location:

  12q13.2

• Reactivity:

  Human/Mouse/Rat

• Cross Reactivity:

  Mouse

• Sequence:

  NLGLDCDEHS SESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGQCEYM FMQKYPHTHL VQQANPRGSA GPCCTPTKMS PINMLYFNDK QQIIYGKIPG MVVDRCGCS

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The ED50 was determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.08-0.10 µg/ml.

• Length:

  109

• Form:

  Lyophilized

• Molecular Weight:

  25 kDa

• Host or Source:

  E. coli