G-CSF

• Description:

  G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Recombinant rat G-CSF is a 21.6 kDa protein consisting of 196 amino acid residues.

• Synonyms:

  Granulocyte Colony-Stimulating Factor, CSF-3, MGI-1G, GM-CSFß, pluripoietin

• NCBI Gene ID:

  25610

• UniProt:

  P97712

• Accession Number:

  NP_058800.1

• Accession Number mRNA:

  NM_017104.1

• Chromosomal Location:

  10q31

• Reactivity:

  Rat

• Cross Reactivity:

  Rat

• Sequence:

  MKKIPLLTVS SLPPSLPLPR SFLLKSLEQV RKIQARNTEL LEQLCATYKL CHPEELVLFG HSLGIPKASL SSCSSQALQQ TKCLSQLHSG LFLYQGLLQA LAGISSELAP TLDMLHLDVD NFATTIWQQM ESLGVAPTVQ PTQSTMPIFT SAFQRRAGGV LVTSYLQSFL ETAHHALHHL PRPAQKHFPE SLFISI

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to stimulate the proliferation of mouse NFS-60 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

• Length:

  196

• Form:

  Lyophilized

• Molecular Weight:

  21.6 kDa

• Host or Source:

  E. coli