FGF-7/KGF

• Description:

  FGF‐7/KGF is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth, and the regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF‐7/KGF is a mitogen factor specific for epithelial cells and keratinocytes. FGF‐7/KGF signals through FGFR 2b. FGF‐7/KGF plays a role in kidney and lung development, as well as in angiogenesis and wound healing. Recombinant Murine FGF‐7/KGF is an 18.9 kDa protein consisting of 164 amino acid residues.

• Synonyms:

  Keratinocyte Growth Factor, Fibroblast Growth Factor-7, HBGF-7

• NCBI Gene ID:

  14178

• UniProt:

  P36363

• Accession Number:

  NP_032034.1

• Accession Number mRNA:

  NM_008008.4

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Sequence:

  MCNDMSPEQT ATSVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNSYNIMEI RTVAVGIVAI KGVESEYYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHSG GEMFVALNQK GIPVKGKKTK KEQKTAHFLP MAIT

• Assay Protocol:

  Centrifuge the vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  ≥ 95% by SDS-PAGE gel and HPLC analyses

• Bioactivity:

  Determined by a cell proliferation assay using 4MBr-5 cells. The expected ED50 for this effect is 0.5-5.0 ng/ml.

• Length:

  164

• Form:

  Lyophilized

• Molecular Weight:

  18.9 kDa

• Storage Conditions:

  The lyophilized protein is stable at room temperature for 3 weeks should be stored desiccated below -18°C. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

• Host or Source:

  E. coli