CD80 / B7-1 Protein (His Tag)

• Description:

  The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

• Synonyms:

  B7 Protein; B71 Protein; Cd28l Protein; Cd80 Protein; Ly-53 Protein; Ly53 Protein; MIC17 Protein; TSA1 Protein

• Accession Number:

  NP_005182.1

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  His-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

• Bioactivity:

  1.Measured by its binding ability in a functional ELISA. Immobilized B7-1 Protein, Human, Recombinant at 2 μg/mL (100 μl/well) can bind CTLA-4 Protein, Human, Recombinant. The EC50 of of CTLA-4 is 35-80 ng/mL. 2.Loaded Recombinant Human CTLA4 / CD152 Protein on ProA Biosensor, can bind Recombinant Human CD80 / B7-1 Protein with an affinity constant of 210 nM as determined in BLI assay (Sartorius Octet RED384) . 3.Loaded Recombinant Human B7-1 Protein, on His1K Biosensor, can bind Recombinant Human CD28 Protein with an affinity constant of 0.46 µM as determined in BLI assay (Sartorius Octet RED384) .

• Length:

  219

• Form:

  Lyophilized or frozen liquid

• Buffer:

  PBS, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  45 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells