BMP-2

• Description :

  BMPs (Bone Morphogenetic Proteins) belong to the TGF-β superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with an osteoconductive carrier such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 appears to play an important role in cardiac morphogenesis, and is expressed in a variety of other tissues, including lung, liver, spleen, prostate, ovary, and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains (monomers) linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease. Recombinant Human BMP-2 derived from CHO cells is a homodimeric glycoprotein that consists of two 114 amino acid polypeptide chains linked by a single disulfide bond. Due to glycosylation, CHO cell-derived Human BMP-2 migrates at an apparent molecular weight of approximately 28-29 kDa by SDS-PAGE analysis under non-reducing conditions.

• Synonyms :

  Bone morphogenetic protein 2; BDA2; BMP2A; bone morphogenetic protein 4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6

• NCBI Gene ID :

  650

• UniProt :

  P12643

• Accession Number :

  NP_001191

• Accession Number mRNA :

  NM_001200

• Chromosomal Location :

  20p12

• Reactivity :

  Human

• Cross Reactivity :

  Human, Mouse, Rat

• Sequence :

  QAKHKQRKRL KSSCKRHPLY VDFSDVGWND WIVAPPGYHA FYCHGECPFP LADHLNSTNH AIVQTLVNSV NSKIPKACCV PTELSAISML YLDENEKVVL KNYQDMVVEG CGCR

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  > 95% by SDS-PAGE & HPLC analyses

• Bioactivity :

  Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 40-100 ng/ml.

• Length :

  114

• Form :

  Lyophilized

• Molecular Weight :

  28-29 kDa

• Host or Source :

  CHO cells

• N Terminal Sequence :

  MQAKHKQ