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Mini Sample Mouse IL-9 (Interleukin 9) ELISA Kit

CAT: 0763-E-MSEL-M0118-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-MSEL-M0118-01Size:24 Tests
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Abbreviation
IL-9
UniProt
P15247
Cross Reactivity
This kit recognizes IL-9 in samples.No significant cross-reactivity or interference between IL-9 and analogues was observed.
Target
IL9; HP40; P40; TCGFIII; TCGF3; P40 T-cell and Mast Cell Growth Factor; P40 Cytokine; T-cell Growth Factor P40
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cell Biology; Signal Transduction; Stem Cells
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-9 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-9 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 43 nm. The OD value is proportional to the concentration of IL-9 . You can calculate the concentration of IL-9 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.95-125 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
1.17 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Detection Instrument
Microplate reader
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-9 concentrations in serum, plasma and other biological fluids.

UniProtKB · P15247

Interleukin-9

IL9_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P15247
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Il9
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
IL-9
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Molecular function

CytokineGrowth factor

PTM

GlycoproteinPyrrolidone carboxylic acid

Cellular component

Secreted

Domain

Signal

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