Mini Sample Mouse IL-1α (Interleukin 1Alpha) ELISA Kit

CAT: 0763-E-MSEL-M0039-01Size: 24 TestsDry Ice: NoHazardous: No

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CAT#:0763-E-MSEL-M0039-01Size:24 Tests

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Abbreviation

IL-1α

UniProt

P01582

Cross Reactivity

This kit recognizes Mouse IL-1α in samples.No significant cross-reactivity or interference between Mouse IL-1α and analogues was observed

Target

IL-1α

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-1α. You can calculate the concentration of Mouse IL-1α in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

3.01 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S2452199X21003510

Citation 02

https://www.nature.com/articles/s41413-019-0056-9

Citation 03

https://www.nature.com/articles/s41413-018-0012-0

Citation 04

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01404-x

Citation 05

https://www.hindawi.com/journals/omcl/2023/9645789/

Citation 06

https://www.mdpi.com/1471758

Citation 07

https://www.sciencedirect.com/science/article/pii/S0378874121011739

Citation 08

https://www.sciencedirect.com/science/article/pii/S0197458018304329

Citation 09

https://www.sciencedirect.com/science/article/pii/S0531556521000887

Citation 10

https://www.sciencedirect.com/science/article/pii/S1567576919328450

Citation 11

https://journals.sagepub.com/doi/abs/10.1177/1559325820920141

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse IL-1α concentrations in serum, plasma and other biological fluids.

UniProtKB · P01582

Interleukin-1 alpha

IL1A_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: P01582
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Il1a
Protein existence: 1: Evidence at protein level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: IL-1 alpha
EC number: —
Processing: Precursor
Secondary accessions: —

Protein keywords

PTM

AcetylationGlycoproteinPhosphoprotein

Molecular function

CytokineMitogenPyrogen

Cellular component

CytoplasmNucleusSecreted

Biological process

Inflammatory response

Technical term

Reference proteome

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