Products for Research Use Only

Mini Sample Mouse IL-2 (Interleukin 2) ELISA Kit

CAT: 0763-E-MSEL-M0036-01Size: 24 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0763-E-MSEL-M0036-01Size:24 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Abbreviation
IL-2
UniProt
P04351
Cross Reactivity
This kit recognizes Mouse IL-2 in samples.No significant cross-reactivity or interference between Mouse IL-2 and analogues was observed
Target
IL-2
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Immunology
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-2. You can calculate the concentration of Mouse IL-2 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.81-500 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
4.69 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.science.org/doi/abs/10.1126/scitranslmed.aaz6667
Citation 02
https://biomaterialsres.biomedcentral.com/articles/10.1186/s40824-022-00297-z
Citation 03
https://www.science.org/doi/abs/10.1126/sciadv.abg8335
Citation 04
https://www.sciencedirect.com/science/article/pii/S2590006423000170
Citation 05
https://www.sciencedirect.com/science/article/pii/S0144861721005981
Citation 06
https://www.sciencedirect.com/science/article/pii/S0141813022014738
Citation 07
https://www.mdpi.com/2076-3921/10/8/1276
Citation 08
https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.7154
Citation 09
https://www.sciencedirect.com/science/article/pii/S0378517322004951
Citation 10
https://www.sciencedirect.com/science/article/pii/S0378874123000600
Citation 11
https://www.sciencedirect.com/science/article/pii/S1567576921009528
Citation 12
https://link.springer.com/article/10.1208/s12249-022-02426-2
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-2 concentrations in serum, plasma and other biological fluids.