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Mini Sample Mouse IL-4 (Interleukin 4) ELISA Kit

CAT: 0763-E-MSEL-M0008-01Size: 10x 96 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-MSEL-M0008-01Size:10x 96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
IL-4
UniProt
P07750
Cross Reactivity
This kit recognizes Mouse IL-4 in samples.No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed
Target
IL-4
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Immunology
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-4. You can calculate the concentration of Mouse IL-4 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
9.38 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0269749122016761
Citation 02
https://www.sciencedirect.com/science/article/pii/S0045653522038267
Citation 03
https://www.sciencedirect.com/science/article/pii/S1742706122003634
Citation 04
https://onlinelibrary.wiley.com/doi/abs/10.1111/imm.13304
Citation 05
https://www.mdpi.com/2037698
Citation 06
https://www.sciencedirect.com/science/article/pii/S0308814621025954
Citation 07
https://www.mdpi.com/2076-3921/10/8/1276
Citation 08
https://pubs.rsc.org/en/content/articlelanding/2022/fo/d2fo00777k
Citation 09
https://www.sciencedirect.com/science/article/pii/S022352342100893X
Citation 10
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355162/
Citation 11
https://onlinelibrary.wiley.com/doi/abs/10.1002/clt2.12114
Citation 12
https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8828541/
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-4 concentrations in serum, plasma and other biological fluids.

UniProtKB · P07750

Interleukin-4

IL4_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P07750
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Il4
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
IL-4
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

AutophagyB-cell activation

Molecular function

CytokineGrowth factor

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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