Potassium Channel Antibody

• Background:

  Voltage-gated K+ channels are important determinants of neuronal membrane excitability, and differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK) channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety on non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes. Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells.

• NCBI Gene ID:

  117105

• Swiss Prot:

  Q63099

• Accession Number:

  Q63099

• Host:

  Rabbit

• Reactivity:

  Rat, Frog

• Immunogen:

  Potassium Channel, Kv2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction, Neuroscience

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Potassium Channel, Kv2.2 Subunit antibody can be stored at -20˚ C. After reconstitution in 50 μ l PBS, the antibody should be aliquot and stored at -20˚ C and is stable at -20˚ C for at least 1 year. Ambient shipment is suitable for both international and domestic.

• Calculated Molecular Weight:

  100

• Applications Notes:

  Potassium Channel antibody can be used for specific immunolabeling of the ~125k Kv 2.2 protein in Western blots. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB) . Rabbit anti-Potassium Channel, Kv2.2 recognizes Kv2.2 in rat and Xenopus. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB.

• Symbol:

  Kcnb2

• NCBI Official Name:

  Potassium voltage gated channel, Shab-related subfamily, member 2

• NCBI Organism:

  Rattus norvegicus

• Background Reference 01:

  Burger, C. and Ribera, A.B. "Xenopus spinal neurons express Kv2 potassium channel transcripts during embryonic development, " J Neurosci. 16 (1996) 1412 - 1421.

• Background Reference 02:

  Pongs, O. "Voltage-gated potassium channels: from hyperexcitability to excitement, " FEBS Lett. 452 (1999) 31-35.

• Background Reference 03:

  Maletic-Savatic, M., Lenn, N.J., and Trimmer, J.S. "Differential spatiotemporal expression of K+ channel polypeptides in rat hippocampal neurons developing in situ and in vitro, " J Neurosci. 15 (1995) 3840-3851.

• Background Reference 04:

  Hwang, P.M., Fotuhi, M., Bredt, D.S., Cunningham, A.M., and Snyder, S.H. "Contrasting immunohistochemical localizations in rat brain of two novel K+ channels of the Shab subfamily, " J. Neurosci., 13 (1993) 1569 - 1576.

• Tested Applications:

  WB, IHC

• Protein ID:

  24418850

• Physical Properties:

  Liquid